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p3504 10g pureblu tm dapi nuclear staining dye bio rad  (Bio-Rad)


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    Bio-Rad p3504 10g pureblu tm dapi nuclear staining dye bio rad
    P3504 10g Pureblu Tm Dapi Nuclear Staining Dye Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p3504+10g+pureblu+tm+dapi+nuclear+staining+dye+bio+rad/PureBlu+DAPI+Nuclear+Staining+Dye/pm41066240-264-226-233
    Average 95 stars, based on 93 article reviews
    p3504 10g pureblu tm dapi nuclear staining dye bio rad - by Bioz Stars, 2026-09
    95/100 stars

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    Electron Microscopy:

    Article Title: Rac1 inhibition prevents axonal cytoskeleton dysfunction in transthyretin amyloid polyneuropathy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-PGP9.5 Abcam Cat# ab108986; RRID:AB_10891773 Custom-made rabbit anti-mouse TTR This paper N/A Rabbit polyclonal anti-human TTR DAKO Cat# A0002; RRID:AB_2335696 Rabbit Polyclonal anti-Desmin Abcam Cat# ab8592 RRID:AB_306653 Mouse Monoclonal anti-Arp3 Santa Cruz Biotechnology Cat# sc-48344; RRID:AB_626700 Rabbit Polyclonal anti-ERK1/2 Cell Signaling Technology Cat# 9102; RRID:AB_330744 Rabbit monoclonal anti-Vinculin Thermo Fisher Cat# 700062; RRID:AB_2532280 Mouse monoclonal anti-Rac1 Abcam Abcam Cat# ab33186; RRID:AB_777598 Mouse Monoclonal anti-RacGAP1 Santa Cruz Biotechnology Cat# sc-271110; RRID:AB_10611939 Rabbit polyclonal anti-Synaptotagmin-2 Synaptic Systems Cat# 105 222; RRID:AB_2619769 Mouse monoclonal anti-βIII tubulin Promega Cat# G7121; RRID:AB_430874 Goat Polyclonal anti-TrkA R&D Systems Cat# AF1056; RRID:AB_2283049 Goat Polyclonal anti-TrkB R&D Systems Cat# AF1494; RRID:AB_2155264 Goat Polyclonal anti-TrkC R&D Systems Cat# AF1404; RRID:AB_2155412 Donkey Anti-Mouse IgG (H + L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A21202; RRID:AB_141607 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 568 Invitrogen Cat# A10042; RRID:AB_2534017 Donkey anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A31571; RRID:AB_162542 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor TM 488 Invitrogen Cat# A21206; RRID:AB_2535792 Goat anti-Guinea Pig IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A21450; RRID:AB_141882 Alexa Fluor® 488 AffiniPure® Donkey Anti-Goat IgG (H + L) Jackson ImmunoResearch Cat# 705-545-147; RRID:AB_2336933 Peroxidase AffiniPure TM Goat Anti-Rabbit IgG (H + L) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Peroxidase AffiniPure TM Goat Anti-Mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003; RRID:AB_10015289 Bacterial and virus strains Lentivirus f(syn)-SYP-GFP Viral Core Facility Charité –Universitaetsmedizin Berlin #BLV-177p Biological samples Human salivary glands Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Human blood samples Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Chemicals, peptides, and recombinant proteins Ammonium chloride (NH4Cl) Merck Cat# 1011450500 Bovine serum albumin (BSA) Sigma Cat# C1889 (Continued on next page) Cell Reports 44, 116411, October 28, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase-IV-S Sigma Cat# C1889 DMEM-F12 medium PAN-Biotech Cat# P04-41150 Epon Electron Microscopy Sciences Cat# 14120 Fetal bovine serum (FBS) Sigma Cat# F9665 Glutaraldehyde Electron Microscopy Sciences Cat# 16316 PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 SDS NZYTech Cat# MB11601 Sodium cacodylate Delta Microscopies Cat# 12300 Triton X-100 Sigma Cat# T9284 Uranyl Acetate Electron Microscopy Sciences Cat# 22400 Neuronal Growth Factor (NGF) Alomone Labs Cat# N-100 L-glutamine Thermo Scientific Cat# 25030024 B-27 TM Supplement Gibco Cat# 17504044 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Skim Milk Powder Merck Cat# 70166-500g Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Sodium chloride (NaCl) Acros Organics Cat# 207790010 Sodium orthovanadate Sigma-Aldrich Cat# S6508-10G Rhodamine-conjugated Phalloidin Thermo Scientific Cat# R415 Protease Inhibitor Mix Merck Cat# GE80-6501-23 RIPA Buffer Merck Cat# R0278-50mL Paraformaldehyde (PFA) Delta Microscopies Cat# GF750170-1010 Toluidin Blue Sigma-Aldrich Cat# 89640-5G Laminin Sigma-Aldrich Cat# L2020 Penicillin/streptomycin Gibco Cat# 15140-122 Poly-L-lysine (PLL) Sigma-Aldrich Cat# P2636 NSC23766 (selective inhibitor of Rac1-GEF interaction) Tocris Bioscience Cat# 2161 5-Fluoro-2 ′ -deoxyuridine (FluoU) Sigma Aldrich Cat# F0503 Neurobasal TM Medium, minus phenol red Invitrogen Cat# 12348-017 Sucrose Merck Cat# 1.07651.1000 Piperazine-1,4-bis(2-ethane-sulfonic acid) (PIPES) Merck Cat# 1.10220.0250 Egtazic acid (EGTA) Sigma-Aldrich Cat# E8145-10G Magnesium chloride (MgCl 2 ) VWR Cat# 1.05833.0250 Fish gelatin Sigma-Aldrich Cat# G7041-100g Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML Ponceau S Sigma-Aldrich Cat# P3504-10G PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Halt TM Protease & Phosphatase Inhibitor Cocktail Thermo Scientific Cat# 78440 RIPA Buffer Merck Cat# R0278-50mL Sucrose Merck Cat# 1.07651.1000 (Continued on next page) 16 Cell Reports 44, 116411, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML D-(+)-Glucose Sigma-Aldrich Cat# G8270-1KG N-2-hydroxyethylpiperazine-N-2ethanesulfonic acid (HEPES) Sigma-Aldrich Cat# H4034-100g Calcium chloride dihydrate (CaCl 2 ⋅2H 2 O) Merck Cat# 1.02382.1000 Magnesium sulfate heptahydrate (MgSO 4 ⋅7H 2 O) Merck Cat# 1.05886.0500 Sodium phosphate dibasic (Na2HPO4) Sigma-Aldrich Cat# S3264-500G Potassium Chloride (KCl) Merck Cat# 1.04935.5000 Collagenase-IV-S Sigma-Aldrich Cat# C1889 Tween® 20 Sigma-Aldrich Cat# P1379-500ML FPS-ZM1 (High affinity antagonist of RAGE) Merck Cat# 553030 Normal Donkey Serum (NDS) Sigma-Aldrich Cat# D9663 DMEM, high glucose, GlutaMAX TM Supplement Gibco Cat# 10569010 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 TRIzol TM Reagent Invitrogen Cat# 15596026 DreamFect TM Gold OZ Biosciences Cat# DG80500 Critical commercial assays QIAamp® DNA Blood Mini Kit QIAGEN Cat# 1043368 Axiom TM Precision Medicine Diversity Array Kit Thermo Scientific Cat# 951962 Zymoclean Gel DNA Recovery Kit Zymo Research Cat# D4001 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat# E0554S PureLink TM RNA Micro Scale Kit Invitrogen Cat# 12183016 NZY First-Strand cDNA Synthesis Kit NZYTech Cat# MB12501 Deposited data Proteomics data ProteomeXchange Consortium via the PRIDE PXD054291 Experimental models: Cell lines

    Staining:

    Article Title: Rac1 inhibition prevents axonal cytoskeleton dysfunction in transthyretin amyloid polyneuropathy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-PGP9.5 Abcam Cat# ab108986; RRID:AB_10891773 Custom-made rabbit anti-mouse TTR This paper N/A Rabbit polyclonal anti-human TTR DAKO Cat# A0002; RRID:AB_2335696 Rabbit Polyclonal anti-Desmin Abcam Cat# ab8592 RRID:AB_306653 Mouse Monoclonal anti-Arp3 Santa Cruz Biotechnology Cat# sc-48344; RRID:AB_626700 Rabbit Polyclonal anti-ERK1/2 Cell Signaling Technology Cat# 9102; RRID:AB_330744 Rabbit monoclonal anti-Vinculin Thermo Fisher Cat# 700062; RRID:AB_2532280 Mouse monoclonal anti-Rac1 Abcam Abcam Cat# ab33186; RRID:AB_777598 Mouse Monoclonal anti-RacGAP1 Santa Cruz Biotechnology Cat# sc-271110; RRID:AB_10611939 Rabbit polyclonal anti-Synaptotagmin-2 Synaptic Systems Cat# 105 222; RRID:AB_2619769 Mouse monoclonal anti-βIII tubulin Promega Cat# G7121; RRID:AB_430874 Goat Polyclonal anti-TrkA R&D Systems Cat# AF1056; RRID:AB_2283049 Goat Polyclonal anti-TrkB R&D Systems Cat# AF1494; RRID:AB_2155264 Goat Polyclonal anti-TrkC R&D Systems Cat# AF1404; RRID:AB_2155412 Donkey Anti-Mouse IgG (H + L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A21202; RRID:AB_141607 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 568 Invitrogen Cat# A10042; RRID:AB_2534017 Donkey anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A31571; RRID:AB_162542 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor TM 488 Invitrogen Cat# A21206; RRID:AB_2535792 Goat anti-Guinea Pig IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A21450; RRID:AB_141882 Alexa Fluor® 488 AffiniPure® Donkey Anti-Goat IgG (H + L) Jackson ImmunoResearch Cat# 705-545-147; RRID:AB_2336933 Peroxidase AffiniPure TM Goat Anti-Rabbit IgG (H + L) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Peroxidase AffiniPure TM Goat Anti-Mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003; RRID:AB_10015289 Bacterial and virus strains Lentivirus f(syn)-SYP-GFP Viral Core Facility Charité –Universitaetsmedizin Berlin #BLV-177p Biological samples Human salivary glands Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Human blood samples Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Chemicals, peptides, and recombinant proteins Ammonium chloride (NH4Cl) Merck Cat# 1011450500 Bovine serum albumin (BSA) Sigma Cat# C1889 (Continued on next page) Cell Reports 44, 116411, October 28, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase-IV-S Sigma Cat# C1889 DMEM-F12 medium PAN-Biotech Cat# P04-41150 Epon Electron Microscopy Sciences Cat# 14120 Fetal bovine serum (FBS) Sigma Cat# F9665 Glutaraldehyde Electron Microscopy Sciences Cat# 16316 PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 SDS NZYTech Cat# MB11601 Sodium cacodylate Delta Microscopies Cat# 12300 Triton X-100 Sigma Cat# T9284 Uranyl Acetate Electron Microscopy Sciences Cat# 22400 Neuronal Growth Factor (NGF) Alomone Labs Cat# N-100 L-glutamine Thermo Scientific Cat# 25030024 B-27 TM Supplement Gibco Cat# 17504044 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Skim Milk Powder Merck Cat# 70166-500g Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Sodium chloride (NaCl) Acros Organics Cat# 207790010 Sodium orthovanadate Sigma-Aldrich Cat# S6508-10G Rhodamine-conjugated Phalloidin Thermo Scientific Cat# R415 Protease Inhibitor Mix Merck Cat# GE80-6501-23 RIPA Buffer Merck Cat# R0278-50mL Paraformaldehyde (PFA) Delta Microscopies Cat# GF750170-1010 Toluidin Blue Sigma-Aldrich Cat# 89640-5G Laminin Sigma-Aldrich Cat# L2020 Penicillin/streptomycin Gibco Cat# 15140-122 Poly-L-lysine (PLL) Sigma-Aldrich Cat# P2636 NSC23766 (selective inhibitor of Rac1-GEF interaction) Tocris Bioscience Cat# 2161 5-Fluoro-2 ′ -deoxyuridine (FluoU) Sigma Aldrich Cat# F0503 Neurobasal TM Medium, minus phenol red Invitrogen Cat# 12348-017 Sucrose Merck Cat# 1.07651.1000 Piperazine-1,4-bis(2-ethane-sulfonic acid) (PIPES) Merck Cat# 1.10220.0250 Egtazic acid (EGTA) Sigma-Aldrich Cat# E8145-10G Magnesium chloride (MgCl 2 ) VWR Cat# 1.05833.0250 Fish gelatin Sigma-Aldrich Cat# G7041-100g Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML Ponceau S Sigma-Aldrich Cat# P3504-10G PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Halt TM Protease & Phosphatase Inhibitor Cocktail Thermo Scientific Cat# 78440 RIPA Buffer Merck Cat# R0278-50mL Sucrose Merck Cat# 1.07651.1000 (Continued on next page) 16 Cell Reports 44, 116411, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML D-(+)-Glucose Sigma-Aldrich Cat# G8270-1KG N-2-hydroxyethylpiperazine-N-2ethanesulfonic acid (HEPES) Sigma-Aldrich Cat# H4034-100g Calcium chloride dihydrate (CaCl 2 ⋅2H 2 O) Merck Cat# 1.02382.1000 Magnesium sulfate heptahydrate (MgSO 4 ⋅7H 2 O) Merck Cat# 1.05886.0500 Sodium phosphate dibasic (Na2HPO4) Sigma-Aldrich Cat# S3264-500G Potassium Chloride (KCl) Merck Cat# 1.04935.5000 Collagenase-IV-S Sigma-Aldrich Cat# C1889 Tween® 20 Sigma-Aldrich Cat# P1379-500ML FPS-ZM1 (High affinity antagonist of RAGE) Merck Cat# 553030 Normal Donkey Serum (NDS) Sigma-Aldrich Cat# D9663 DMEM, high glucose, GlutaMAX TM Supplement Gibco Cat# 10569010 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 TRIzol TM Reagent Invitrogen Cat# 15596026 DreamFect TM Gold OZ Biosciences Cat# DG80500 Critical commercial assays QIAamp® DNA Blood Mini Kit QIAGEN Cat# 1043368 Axiom TM Precision Medicine Diversity Array Kit Thermo Scientific Cat# 951962 Zymoclean Gel DNA Recovery Kit Zymo Research Cat# D4001 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat# E0554S PureLink TM RNA Micro Scale Kit Invitrogen Cat# 12183016 NZY First-Strand cDNA Synthesis Kit NZYTech Cat# MB12501 Deposited data Proteomics data ProteomeXchange Consortium via the PRIDE PXD054291 Experimental models: Cell lines

    Milk:

    Article Title: Rac1 inhibition prevents axonal cytoskeleton dysfunction in transthyretin amyloid polyneuropathy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-PGP9.5 Abcam Cat# ab108986; RRID:AB_10891773 Custom-made rabbit anti-mouse TTR This paper N/A Rabbit polyclonal anti-human TTR DAKO Cat# A0002; RRID:AB_2335696 Rabbit Polyclonal anti-Desmin Abcam Cat# ab8592 RRID:AB_306653 Mouse Monoclonal anti-Arp3 Santa Cruz Biotechnology Cat# sc-48344; RRID:AB_626700 Rabbit Polyclonal anti-ERK1/2 Cell Signaling Technology Cat# 9102; RRID:AB_330744 Rabbit monoclonal anti-Vinculin Thermo Fisher Cat# 700062; RRID:AB_2532280 Mouse monoclonal anti-Rac1 Abcam Abcam Cat# ab33186; RRID:AB_777598 Mouse Monoclonal anti-RacGAP1 Santa Cruz Biotechnology Cat# sc-271110; RRID:AB_10611939 Rabbit polyclonal anti-Synaptotagmin-2 Synaptic Systems Cat# 105 222; RRID:AB_2619769 Mouse monoclonal anti-βIII tubulin Promega Cat# G7121; RRID:AB_430874 Goat Polyclonal anti-TrkA R&D Systems Cat# AF1056; RRID:AB_2283049 Goat Polyclonal anti-TrkB R&D Systems Cat# AF1494; RRID:AB_2155264 Goat Polyclonal anti-TrkC R&D Systems Cat# AF1404; RRID:AB_2155412 Donkey Anti-Mouse IgG (H + L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A21202; RRID:AB_141607 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 568 Invitrogen Cat# A10042; RRID:AB_2534017 Donkey anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A31571; RRID:AB_162542 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor TM 488 Invitrogen Cat# A21206; RRID:AB_2535792 Goat anti-Guinea Pig IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A21450; RRID:AB_141882 Alexa Fluor® 488 AffiniPure® Donkey Anti-Goat IgG (H + L) Jackson ImmunoResearch Cat# 705-545-147; RRID:AB_2336933 Peroxidase AffiniPure TM Goat Anti-Rabbit IgG (H + L) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Peroxidase AffiniPure TM Goat Anti-Mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003; RRID:AB_10015289 Bacterial and virus strains Lentivirus f(syn)-SYP-GFP Viral Core Facility Charité –Universitaetsmedizin Berlin #BLV-177p Biological samples Human salivary glands Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Human blood samples Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Chemicals, peptides, and recombinant proteins Ammonium chloride (NH4Cl) Merck Cat# 1011450500 Bovine serum albumin (BSA) Sigma Cat# C1889 (Continued on next page) Cell Reports 44, 116411, October 28, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase-IV-S Sigma Cat# C1889 DMEM-F12 medium PAN-Biotech Cat# P04-41150 Epon Electron Microscopy Sciences Cat# 14120 Fetal bovine serum (FBS) Sigma Cat# F9665 Glutaraldehyde Electron Microscopy Sciences Cat# 16316 PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 SDS NZYTech Cat# MB11601 Sodium cacodylate Delta Microscopies Cat# 12300 Triton X-100 Sigma Cat# T9284 Uranyl Acetate Electron Microscopy Sciences Cat# 22400 Neuronal Growth Factor (NGF) Alomone Labs Cat# N-100 L-glutamine Thermo Scientific Cat# 25030024 B-27 TM Supplement Gibco Cat# 17504044 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Skim Milk Powder Merck Cat# 70166-500g Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Sodium chloride (NaCl) Acros Organics Cat# 207790010 Sodium orthovanadate Sigma-Aldrich Cat# S6508-10G Rhodamine-conjugated Phalloidin Thermo Scientific Cat# R415 Protease Inhibitor Mix Merck Cat# GE80-6501-23 RIPA Buffer Merck Cat# R0278-50mL Paraformaldehyde (PFA) Delta Microscopies Cat# GF750170-1010 Toluidin Blue Sigma-Aldrich Cat# 89640-5G Laminin Sigma-Aldrich Cat# L2020 Penicillin/streptomycin Gibco Cat# 15140-122 Poly-L-lysine (PLL) Sigma-Aldrich Cat# P2636 NSC23766 (selective inhibitor of Rac1-GEF interaction) Tocris Bioscience Cat# 2161 5-Fluoro-2 ′ -deoxyuridine (FluoU) Sigma Aldrich Cat# F0503 Neurobasal TM Medium, minus phenol red Invitrogen Cat# 12348-017 Sucrose Merck Cat# 1.07651.1000 Piperazine-1,4-bis(2-ethane-sulfonic acid) (PIPES) Merck Cat# 1.10220.0250 Egtazic acid (EGTA) Sigma-Aldrich Cat# E8145-10G Magnesium chloride (MgCl 2 ) VWR Cat# 1.05833.0250 Fish gelatin Sigma-Aldrich Cat# G7041-100g Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML Ponceau S Sigma-Aldrich Cat# P3504-10G PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Halt TM Protease & Phosphatase Inhibitor Cocktail Thermo Scientific Cat# 78440 RIPA Buffer Merck Cat# R0278-50mL Sucrose Merck Cat# 1.07651.1000 (Continued on next page) 16 Cell Reports 44, 116411, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML D-(+)-Glucose Sigma-Aldrich Cat# G8270-1KG N-2-hydroxyethylpiperazine-N-2ethanesulfonic acid (HEPES) Sigma-Aldrich Cat# H4034-100g Calcium chloride dihydrate (CaCl 2 ⋅2H 2 O) Merck Cat# 1.02382.1000 Magnesium sulfate heptahydrate (MgSO 4 ⋅7H 2 O) Merck Cat# 1.05886.0500 Sodium phosphate dibasic (Na2HPO4) Sigma-Aldrich Cat# S3264-500G Potassium Chloride (KCl) Merck Cat# 1.04935.5000 Collagenase-IV-S Sigma-Aldrich Cat# C1889 Tween® 20 Sigma-Aldrich Cat# P1379-500ML FPS-ZM1 (High affinity antagonist of RAGE) Merck Cat# 553030 Normal Donkey Serum (NDS) Sigma-Aldrich Cat# D9663 DMEM, high glucose, GlutaMAX TM Supplement Gibco Cat# 10569010 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 TRIzol TM Reagent Invitrogen Cat# 15596026 DreamFect TM Gold OZ Biosciences Cat# DG80500 Critical commercial assays QIAamp® DNA Blood Mini Kit QIAGEN Cat# 1043368 Axiom TM Precision Medicine Diversity Array Kit Thermo Scientific Cat# 951962 Zymoclean Gel DNA Recovery Kit Zymo Research Cat# D4001 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat# E0554S PureLink TM RNA Micro Scale Kit Invitrogen Cat# 12183016 NZY First-Strand cDNA Synthesis Kit NZYTech Cat# MB12501 Deposited data Proteomics data ProteomeXchange Consortium via the PRIDE PXD054291 Experimental models: Cell lines

    Western Blot:

    Article Title: Rac1 inhibition prevents axonal cytoskeleton dysfunction in transthyretin amyloid polyneuropathy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-PGP9.5 Abcam Cat# ab108986; RRID:AB_10891773 Custom-made rabbit anti-mouse TTR This paper N/A Rabbit polyclonal anti-human TTR DAKO Cat# A0002; RRID:AB_2335696 Rabbit Polyclonal anti-Desmin Abcam Cat# ab8592 RRID:AB_306653 Mouse Monoclonal anti-Arp3 Santa Cruz Biotechnology Cat# sc-48344; RRID:AB_626700 Rabbit Polyclonal anti-ERK1/2 Cell Signaling Technology Cat# 9102; RRID:AB_330744 Rabbit monoclonal anti-Vinculin Thermo Fisher Cat# 700062; RRID:AB_2532280 Mouse monoclonal anti-Rac1 Abcam Abcam Cat# ab33186; RRID:AB_777598 Mouse Monoclonal anti-RacGAP1 Santa Cruz Biotechnology Cat# sc-271110; RRID:AB_10611939 Rabbit polyclonal anti-Synaptotagmin-2 Synaptic Systems Cat# 105 222; RRID:AB_2619769 Mouse monoclonal anti-βIII tubulin Promega Cat# G7121; RRID:AB_430874 Goat Polyclonal anti-TrkA R&D Systems Cat# AF1056; RRID:AB_2283049 Goat Polyclonal anti-TrkB R&D Systems Cat# AF1494; RRID:AB_2155264 Goat Polyclonal anti-TrkC R&D Systems Cat# AF1404; RRID:AB_2155412 Donkey Anti-Mouse IgG (H + L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A21202; RRID:AB_141607 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 568 Invitrogen Cat# A10042; RRID:AB_2534017 Donkey anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A31571; RRID:AB_162542 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor TM 488 Invitrogen Cat# A21206; RRID:AB_2535792 Goat anti-Guinea Pig IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A21450; RRID:AB_141882 Alexa Fluor® 488 AffiniPure® Donkey Anti-Goat IgG (H + L) Jackson ImmunoResearch Cat# 705-545-147; RRID:AB_2336933 Peroxidase AffiniPure TM Goat Anti-Rabbit IgG (H + L) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Peroxidase AffiniPure TM Goat Anti-Mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003; RRID:AB_10015289 Bacterial and virus strains Lentivirus f(syn)-SYP-GFP Viral Core Facility Charité –Universitaetsmedizin Berlin #BLV-177p Biological samples Human salivary glands Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Human blood samples Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Chemicals, peptides, and recombinant proteins Ammonium chloride (NH4Cl) Merck Cat# 1011450500 Bovine serum albumin (BSA) Sigma Cat# C1889 (Continued on next page) Cell Reports 44, 116411, October 28, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase-IV-S Sigma Cat# C1889 DMEM-F12 medium PAN-Biotech Cat# P04-41150 Epon Electron Microscopy Sciences Cat# 14120 Fetal bovine serum (FBS) Sigma Cat# F9665 Glutaraldehyde Electron Microscopy Sciences Cat# 16316 PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 SDS NZYTech Cat# MB11601 Sodium cacodylate Delta Microscopies Cat# 12300 Triton X-100 Sigma Cat# T9284 Uranyl Acetate Electron Microscopy Sciences Cat# 22400 Neuronal Growth Factor (NGF) Alomone Labs Cat# N-100 L-glutamine Thermo Scientific Cat# 25030024 B-27 TM Supplement Gibco Cat# 17504044 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Skim Milk Powder Merck Cat# 70166-500g Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Sodium chloride (NaCl) Acros Organics Cat# 207790010 Sodium orthovanadate Sigma-Aldrich Cat# S6508-10G Rhodamine-conjugated Phalloidin Thermo Scientific Cat# R415 Protease Inhibitor Mix Merck Cat# GE80-6501-23 RIPA Buffer Merck Cat# R0278-50mL Paraformaldehyde (PFA) Delta Microscopies Cat# GF750170-1010 Toluidin Blue Sigma-Aldrich Cat# 89640-5G Laminin Sigma-Aldrich Cat# L2020 Penicillin/streptomycin Gibco Cat# 15140-122 Poly-L-lysine (PLL) Sigma-Aldrich Cat# P2636 NSC23766 (selective inhibitor of Rac1-GEF interaction) Tocris Bioscience Cat# 2161 5-Fluoro-2 ′ -deoxyuridine (FluoU) Sigma Aldrich Cat# F0503 Neurobasal TM Medium, minus phenol red Invitrogen Cat# 12348-017 Sucrose Merck Cat# 1.07651.1000 Piperazine-1,4-bis(2-ethane-sulfonic acid) (PIPES) Merck Cat# 1.10220.0250 Egtazic acid (EGTA) Sigma-Aldrich Cat# E8145-10G Magnesium chloride (MgCl 2 ) VWR Cat# 1.05833.0250 Fish gelatin Sigma-Aldrich Cat# G7041-100g Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML Ponceau S Sigma-Aldrich Cat# P3504-10G PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Halt TM Protease & Phosphatase Inhibitor Cocktail Thermo Scientific Cat# 78440 RIPA Buffer Merck Cat# R0278-50mL Sucrose Merck Cat# 1.07651.1000 (Continued on next page) 16 Cell Reports 44, 116411, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML D-(+)-Glucose Sigma-Aldrich Cat# G8270-1KG N-2-hydroxyethylpiperazine-N-2ethanesulfonic acid (HEPES) Sigma-Aldrich Cat# H4034-100g Calcium chloride dihydrate (CaCl 2 ⋅2H 2 O) Merck Cat# 1.02382.1000 Magnesium sulfate heptahydrate (MgSO 4 ⋅7H 2 O) Merck Cat# 1.05886.0500 Sodium phosphate dibasic (Na2HPO4) Sigma-Aldrich Cat# S3264-500G Potassium Chloride (KCl) Merck Cat# 1.04935.5000 Collagenase-IV-S Sigma-Aldrich Cat# C1889 Tween® 20 Sigma-Aldrich Cat# P1379-500ML FPS-ZM1 (High affinity antagonist of RAGE) Merck Cat# 553030 Normal Donkey Serum (NDS) Sigma-Aldrich Cat# D9663 DMEM, high glucose, GlutaMAX TM Supplement Gibco Cat# 10569010 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 TRIzol TM Reagent Invitrogen Cat# 15596026 DreamFect TM Gold OZ Biosciences Cat# DG80500 Critical commercial assays QIAamp® DNA Blood Mini Kit QIAGEN Cat# 1043368 Axiom TM Precision Medicine Diversity Array Kit Thermo Scientific Cat# 951962 Zymoclean Gel DNA Recovery Kit Zymo Research Cat# D4001 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat# E0554S PureLink TM RNA Micro Scale Kit Invitrogen Cat# 12183016 NZY First-Strand cDNA Synthesis Kit NZYTech Cat# MB12501 Deposited data Proteomics data ProteomeXchange Consortium via the PRIDE PXD054291 Experimental models: Cell lines

    Protease Inhibitor:

    Article Title: Rac1 inhibition prevents axonal cytoskeleton dysfunction in transthyretin amyloid polyneuropathy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-PGP9.5 Abcam Cat# ab108986; RRID:AB_10891773 Custom-made rabbit anti-mouse TTR This paper N/A Rabbit polyclonal anti-human TTR DAKO Cat# A0002; RRID:AB_2335696 Rabbit Polyclonal anti-Desmin Abcam Cat# ab8592 RRID:AB_306653 Mouse Monoclonal anti-Arp3 Santa Cruz Biotechnology Cat# sc-48344; RRID:AB_626700 Rabbit Polyclonal anti-ERK1/2 Cell Signaling Technology Cat# 9102; RRID:AB_330744 Rabbit monoclonal anti-Vinculin Thermo Fisher Cat# 700062; RRID:AB_2532280 Mouse monoclonal anti-Rac1 Abcam Abcam Cat# ab33186; RRID:AB_777598 Mouse Monoclonal anti-RacGAP1 Santa Cruz Biotechnology Cat# sc-271110; RRID:AB_10611939 Rabbit polyclonal anti-Synaptotagmin-2 Synaptic Systems Cat# 105 222; RRID:AB_2619769 Mouse monoclonal anti-βIII tubulin Promega Cat# G7121; RRID:AB_430874 Goat Polyclonal anti-TrkA R&D Systems Cat# AF1056; RRID:AB_2283049 Goat Polyclonal anti-TrkB R&D Systems Cat# AF1494; RRID:AB_2155264 Goat Polyclonal anti-TrkC R&D Systems Cat# AF1404; RRID:AB_2155412 Donkey Anti-Mouse IgG (H + L) Antibody, Alexa Fluor 488 Conjugated Invitrogen Cat# A21202; RRID:AB_141607 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 568 Invitrogen Cat# A10042; RRID:AB_2534017 Donkey anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A31571; RRID:AB_162542 Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor TM 488 Invitrogen Cat# A21206; RRID:AB_2535792 Goat anti-Guinea Pig IgG (H + L) Secondary Antibody, Alexa Fluor TM 647 Invitrogen Cat# A21450; RRID:AB_141882 Alexa Fluor® 488 AffiniPure® Donkey Anti-Goat IgG (H + L) Jackson ImmunoResearch Cat# 705-545-147; RRID:AB_2336933 Peroxidase AffiniPure TM Goat Anti-Rabbit IgG (H + L) Jackson ImmunoResearch Cat# 111-035-003; RRID:AB_2313567 Peroxidase AffiniPure TM Goat Anti-Mouse IgG (H + L) Jackson ImmunoResearch Cat# 115-035-003; RRID:AB_10015289 Bacterial and virus strains Lentivirus f(syn)-SYP-GFP Viral Core Facility Charité –Universitaetsmedizin Berlin #BLV-177p Biological samples Human salivary glands Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Human blood samples Unidade Corino de Andrade at Centro Hospitalar Universitá rio de Santo Antó nio (UCA-CHUdSA) N/A Chemicals, peptides, and recombinant proteins Ammonium chloride (NH4Cl) Merck Cat# 1011450500 Bovine serum albumin (BSA) Sigma Cat# C1889 (Continued on next page) Cell Reports 44, 116411, October 28, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase-IV-S Sigma Cat# C1889 DMEM-F12 medium PAN-Biotech Cat# P04-41150 Epon Electron Microscopy Sciences Cat# 14120 Fetal bovine serum (FBS) Sigma Cat# F9665 Glutaraldehyde Electron Microscopy Sciences Cat# 16316 PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 SDS NZYTech Cat# MB11601 Sodium cacodylate Delta Microscopies Cat# 12300 Triton X-100 Sigma Cat# T9284 Uranyl Acetate Electron Microscopy Sciences Cat# 22400 Neuronal Growth Factor (NGF) Alomone Labs Cat# N-100 L-glutamine Thermo Scientific Cat# 25030024 B-27 TM Supplement Gibco Cat# 17504044 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Skim Milk Powder Merck Cat# 70166-500g Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Sodium chloride (NaCl) Acros Organics Cat# 207790010 Sodium orthovanadate Sigma-Aldrich Cat# S6508-10G Rhodamine-conjugated Phalloidin Thermo Scientific Cat# R415 Protease Inhibitor Mix Merck Cat# GE80-6501-23 RIPA Buffer Merck Cat# R0278-50mL Paraformaldehyde (PFA) Delta Microscopies Cat# GF750170-1010 Toluidin Blue Sigma-Aldrich Cat# 89640-5G Laminin Sigma-Aldrich Cat# L2020 Penicillin/streptomycin Gibco Cat# 15140-122 Poly-L-lysine (PLL) Sigma-Aldrich Cat# P2636 NSC23766 (selective inhibitor of Rac1-GEF interaction) Tocris Bioscience Cat# 2161 5-Fluoro-2 ′ -deoxyuridine (FluoU) Sigma Aldrich Cat# F0503 Neurobasal TM Medium, minus phenol red Invitrogen Cat# 12348-017 Sucrose Merck Cat# 1.07651.1000 Piperazine-1,4-bis(2-ethane-sulfonic acid) (PIPES) Merck Cat# 1.10220.0250 Egtazic acid (EGTA) Sigma-Aldrich Cat# E8145-10G Magnesium chloride (MgCl 2 ) VWR Cat# 1.05833.0250 Fish gelatin Sigma-Aldrich Cat# G7041-100g Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML Ponceau S Sigma-Aldrich Cat# P3504-10G PureBlu TM DAPI Nuclear Staining Dye Bio-Rad Cat# 1351303 Osmium tetroxide Electron Microscopy Sciences Cat# 19190 Immobilon Crescendo Western HRP substrate Merck Cat# WBLUR0500 Halt TM Protease & Phosphatase Inhibitor Cocktail Thermo Scientific Cat# 78440 RIPA Buffer Merck Cat# R0278-50mL Sucrose Merck Cat# 1.07651.1000 (Continued on next page) 16 Cell Reports 44, 116411, October 28, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Glutathione High Capacity Magnetic Agarose Beads Merck Cat# G0924-1ML D-(+)-Glucose Sigma-Aldrich Cat# G8270-1KG N-2-hydroxyethylpiperazine-N-2ethanesulfonic acid (HEPES) Sigma-Aldrich Cat# H4034-100g Calcium chloride dihydrate (CaCl 2 ⋅2H 2 O) Merck Cat# 1.02382.1000 Magnesium sulfate heptahydrate (MgSO 4 ⋅7H 2 O) Merck Cat# 1.05886.0500 Sodium phosphate dibasic (Na2HPO4) Sigma-Aldrich Cat# S3264-500G Potassium Chloride (KCl) Merck Cat# 1.04935.5000 Collagenase-IV-S Sigma-Aldrich Cat# C1889 Tween® 20 Sigma-Aldrich Cat# P1379-500ML FPS-ZM1 (High affinity antagonist of RAGE) Merck Cat# 553030 Normal Donkey Serum (NDS) Sigma-Aldrich Cat# D9663 DMEM, high glucose, GlutaMAX TM Supplement Gibco Cat# 10569010 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 TRIzol TM Reagent Invitrogen Cat# 15596026 DreamFect TM Gold OZ Biosciences Cat# DG80500 Critical commercial assays QIAamp® DNA Blood Mini Kit QIAGEN Cat# 1043368 Axiom TM Precision Medicine Diversity Array Kit Thermo Scientific Cat# 951962 Zymoclean Gel DNA Recovery Kit Zymo Research Cat# D4001 Q5® Site-Directed Mutagenesis Kit New England Biolabs Cat# E0554S PureLink TM RNA Micro Scale Kit Invitrogen Cat# 12183016 NZY First-Strand cDNA Synthesis Kit NZYTech Cat# MB12501 Deposited data Proteomics data ProteomeXchange Consortium via the PRIDE PXD054291 Experimental models: Cell lines



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